2 lines on first response but negative digital

Can a Faint Line Be Negative?

Pregnancy tests have one goal: to detect hCG. When your test shows a faint line, it means that your body is detecting some hCG, which typically indicates pregnancy. Urine tests should display a positive test when 25 mIU/ml hCG is detected, according to a German study on pregnancy tests.

However, it is possible to detect a thin line that disappears. Additionally, you might start your period soon after spotting a thin line. This might indicate an extremely early loss, such as a chemical pregnancy.

Beyond early pregnancy loss, there are other circumstances that could cause a faint line to appear even if you are not actually pregnant. These situations include:

  • Hormonal imbalances such as PCOS: Sometimes ovarian cysts may release hormones (including hCG) even if pregnancy isn’t achieved.
  • Fertility medication: Some fertility medications may trigger positive results before you’re actually pregnant. If you’re currently going through fertility treatments, it’s a good idea to take a pregnancy test only when advised by your provider.
  • Recent miscarriage: If your hCG levels have not returned to 0 mIU/ml hCG, it’s possible that a pregnancy test is still detecting residual hCG.
  • Menopause: Early menopause can also trigger higher than normal hCG levels, which could lead to a false positive.
  • Illnesses: Specifically, medications used for Parkinson’s disease and epilepsy can trigger a false positive.
  • hCG diet: The hCG diet is a weight loss diet that incorporates this hormone into the treatment plan. Not surprisingly, if you’re on the hCG diet, you’ll likely get positive pregnancy results since the test is picking up those hormone levels.
  • What Does a Faint Line on a Pregnancy Test Look Like?

    Different tests produce different results for a faint line. A plus sign or two parallel lines are typically used on pregnancy tests to indicate pregnancy.

  • If you take a test with the plus sign, you’ll be looking for one dark vertical line (the control line) and one faint horizontal line crossing it.
  • If you take a test with parallel lines, you’ll be looking for one darker line next to one faint line.
  • The faint line may be so faint it’s barely visible if you test early (before you’ve even missed your period), almost like a tiny, tiny smudge. Typically, the line gets darker as you move along. The following illustrates how this progression looks:

    How Does a Pregnancy Test Work?

    To give you a quick overview, at-home pregnancy tests look for the hormone Human Chorionic Gonadotropin, or hCG, in your urine. After fertilization, hCG levels quickly rise in your body. If hCG is found, the lines on a pregnancy test take on color.

    An at-home First Response™ pregnancy test works like this:

  • Special reagents are deposited at 2 locations on a pregnancy test strip, forming the “control line” and the “test line.”
  • These test reagents may appear as off-white/slightly gray lines on the test strip – before or after use. In a First Response™ pregnancy test, these reagents are gold nanoparticles, which appear pink in color. They are responsible for turning the lines pink once the test is used.
  • If no hCG is detected in your urine, only the control line will appear pink colored. If hCG is present in the urine, both the test line and control line will appear pink. Two pink lines indicate a positive result, even if one line is fainter than the other.
  • You can test up to 6 days earlier with some tests, such as First ResponseTM Early Result Pregnancy Tests, because they are highly sensitive and can identify lower levels of hCG. *.


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