Pregnancy Test Results After a Frozen Embryo Transfer

Day by day of the IVF embryo transfer process

According to NYU Langone Fertility, the following occurs each day after IVF embryo transfer:

  • Day 1: The blastocyst begins to hatch out of its shell.
  • Day 2: The blastocyst continues to hatch out of its shell and begins to attach itself to the uterus.
  • Day 3: The blastocyst attaches deeper into the uterine lining, beginning implantation.
  • Day 4: Implantation continues.
  • Day 5: Implantation is complete. Cells that eventually become the placenta and fetus have begun to develop.
  • Day 6: Human chorionic gonadotropin (hCG), the hormone that signals a developing pregnancy, starts to enter the bloodstream.
  • Days 7 and 8: Fetal development continues, and hCG continues to be secreted.
  • Day 9: Levels of hCG are now high enough in maternal blood to detect pregnancy using a blood test.
  • With the ability to detect hCG at just 10 mIU/ml, the at-home Natalist Pregnancy Test is a very early test. Some customers will start to notice a faint line less than a week after the transfer, as you can see in the customer photos below.

    So, what does a pregnancy test that is positive following an embryo transfer look like?

    You’ll probably want to perform a pregnancy test following an IVF embryo transfer. A positive pregnancy test result, however, doesn’t always appear clearly and can appear as follows depending on how soon you test:

    1. Here is an illustration of a test result with a developing faint positive line (6dp5dt):

    Positive Pregnancy Test 7 Days Post Fet

    2. Another instance of a slender line starting to form on a positive test result is shown here: 6dp5dt

    Positive Pregnancy Test 7 Days Post Fet

    3. Here is an example positive test result 7dp5dt:

    Positive Pregnancy Test 7 Days Post Fet

    4. Another illustration of a positive test result from 7dp5dt is shown here:

    Positive Pregnancy Test 7 Days Post Fet

    5. Here is a pregnancy test from 8dp5dt. This mother tested at 10:56 in the morning, so her urine wasn’t the first of the day and might have been darker if she had done so earlier:

    Positive Pregnancy Test 7 Days Post Fet

    6. Here are the three successively positive results from an 8dp5dt, 11dp5dt, and 14dp5dt mama:

    Positive Pregnancy Test 7 Days Post Fet

    The earlier you test, the lighter the line will be. The line will become gradually darker each day if you are pregnant. Some things to remember when testing:

  • It’s best to test first thing in the morning when your urine is most concentrated, but we don’t blame you for testing more than once in a day!
  • Pee directly on the absorbent tip (everything below the arrow) until it’s completely wet or dip in a cup of urine.
  • Write the date and time on the test so you can compare progress across days.
  • You’ll want to make sure you aren’t taking any medication that would give you a false positive. If you don’t know which medications may do so, ask your nurse coordinator!
  • Unluckily, a lot of the early pregnancy symptoms resemble the negative effects of IVF drugs. This includes fatigue, bloating, sore breasts, cramping, and spotting. High hormone levels may result from a successful pregnancy or from taking medicine. Some pregnant women experience these symptoms, while others experience them as a result of their medications. And others have no symptoms at all. This makes predicting pregnancy difficult from symptom tracking alone. An early result pregnancy test or beta hCG test is the best way to determine whether you are pregnant.

    We’re sending sticky thoughts your way in celebration of your IVF transfer.

    Next up:

    Natalist’s founder and infertility activist Halle Tecco Her struggle to conceive, which involved numerous rounds of IVF, motivated her to found Natalist and support other women on their journey. Sign up for her IVF newsletter here .

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    How Early Can You Test After an Embryo Transfer?

    We’ll just cut to the chase: the most accurate time to take a pregnancy test after IVF is 7 days after a day-5 embryo transfer. Why? After a day-5 embryo transfer, it takes a few days for the embryo to safely implant into the endometrial lining of your uterus. Once the embryo has implanted, your body will begin producing hCG, otherwise known as “the pregnancy hormone.” This is the hormone that at-home pregnancy tests detect in your urine.

    Some early at-home pregnancy tests may be able to identify pregnancy as early as 5 days after the transfer, depending on when implantation takes place. Use a high-sensitivity pregnancy test for this, which must detect hCG at a threshold of 25 mIU/mL or less.

    Usually, your fertility clinic will schedule an hCG beta blood test for 8 or 9 days post-embryo-transfer, which studies have shown yields very predictive results for successful pregnancies of both fresh and frozen embryo transfers. This test will give you the most accurate results, as it measures the specific level of hCG in the blood.

    What is the Best Pregnancy Test for Early Detection?

    For a self-proclaimed pee-on-a-stick addict, the best type of early detection at-home pregnancy test is one that has high sensitivity and can detect hCG at a threshold of 25 mIU/mL or less. The First Response Early Result Pregnancy Test detects hCG at 25 mIU/mL, and Natalist’s Pregnancy Test detects hCG as low as 10 mIU/mL.

    We advise performing a test every day starting five days after your transfer if you choose to test at home. The positive result should get darker every day as your hCG levels rise, and the test results will be more accurate the closer you are to your beta hCG blood test.

    Essential facts to know about your IVF pregnancy test

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